@prefix dcat: . @prefix dct: . @prefix foaf: . @prefix locn: . @prefix schema1: . @prefix xsd: . a dcat:Dataset ; dct:creator [ a foaf:Person ; foaf:familyName "Heckenhauer" ; foaf:givenName "Jacqueline" ; foaf:member [ a foaf:Organization ; foaf:name "Senckenberg Gesellschaft für Naturforschung" ] ] ; dct:description """Aquatic insects have been neglected in genomic studies. However, they exhibit a suite of ecologically relevant key innovations and adaptive traits, the evolution and genetic background of which remain poorly understood. This project is designed to fill this gap by generating and analyzing genomic data to study the evolution of adhesive underwater silk in Trichoptera (caddisflies) and other (semi-) aquatic arthropods. Caddisflies exhibit the greatest diversity of underwater silk uses. This exciting key innovation has potentially facilitated their radiation across a multitude of different aquatic environments. Further, the unique properties of under water silk (polymerization in aquatic environment, enormous tensile strength, elasticity) makes this system interest- ing for applied sciences. Using a comparative genomics framework and targeting mechanisms such as gene family expansion, selection, presence/absence of genes and variation in gene sequences (e.g. repeat motifs in important silk gene clusters), the project aims to uncover the genomic basis of the evolution of genes and gene families encoding for silk phenotypes in Trichoptera and other freshwater arthropods. Further, by looking at different developmental stages, genetic modulation and regulation of the different properties of silk will be investigated, i.e., the role of gene expression and post-transcriptional modifications (e.g., alternative splicing) and potential methylation patterns in silk genes will be examined. Understanding the genomic evolution and molecular mechanisms of silk production will not only address questions regarding molecular adaptations responsible for the diversification in aquatic environments but also lay the foundation to gauge the potential of underwater silk for biomedical and biotechnological applications.\r \r The German Research Foundation (Deutsche Forschungsgemeinschaft) priority program “The genomic basis of evolutionary innovations” (SPP2349—Project No. 502865717 awarded to J.H.) enabled this research.\r \r """ ; dct:identifier "3b63501c-382f-4247-89b7-6ff2218ae6c4" ; dct:isReferencedBy , , , , , , ; dct:issued "2026-04-20T12:50:48.177178"^^xsd:dateTime ; dct:modified "2026-04-21T10:11:49.492712"^^xsd:dateTime ; dct:publisher ; dct:spatial [ a dct:location ; dct:locality """Germany\r North America""" ; locn:geometry "{\"type\": \"Point\", \"coordinates\": [null, null]}"^^ ] ; dct:title "Evolution of underwater silk in caddisflies (Insecta:Trichoptera) and other freshwater arthropods" ; dcat:distribution ; dcat:keyword "Trichoptera", "evolution", "genome assembly", "h-fibroin", "silk genes", "transposable elements" . a dcat:Distribution ; dct:description """Data Availability Statement for "Heckenhauer, J., C.Gerheim, G.Jijon, S. U.Pauls, R. W.Wisseman, and P. B.Frandsen. 2026. “Genomics of Caddisfly (Insecta: Trichoptera) Species Associated With Terrestrial Habitats.” Ecology and Evolution16, no. 4: e73327. https://doi.org/10.1002/ece3.73327":\r \r Sample information (E. pusilla: SAMN49671951; P. rivularis: SAMN49671952), raw PacBio HiFi sequencing reads (E. pusilla: SRR36479557; P. rivularis: SRR36482349), and primary assemblies (E. pusilla: JBPQUU000000000; P. rivularis: JBPQUV000000000) have been uploaded to the National Center for Biotechnology Information (NCBI, https://www.ncbi.nlm.nih.gov/). Sequences of primary and alternate h-fibroins have been uploaded to GenBank (https://www.ncbi.nlm.nih.gov/genbank/) at PX673985 and PX673986 (E. pusilla) and PX673987 and PX673988 (P. rivularis). Alternate genome assemblies, functional and structural annotations of the genomes, mitogenomes, repeat annotations and files relevant for phylogenetic inferences are found at: https://figshare.com/s/732ad6f0f7c6a0fa0f74 (10.6084/m9.figshare.30834434).\r \r Appendix S1: Repetitive DNA content in Limnephilidae: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fece3.73327&file=ece373327-sup-0001-AppendixS1.xlsx\r \r Appendix S2: Amino acid composition of the major silk gene h-fibroin: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fece3.73327&file=ece373327-sup-0002-AppendixS2.xlsx\r \r Supplementary Material: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fece3.73327&file=ece373327-sup-0003-Supinfo.pdf\r \r Table S1: Software tools and commands used to perform analyses of this study. All files are available at National Center for Biotechnology Information (NCBI, https://www.ncbi.nlm.nih.gov/) or at https://figshare.com/s/732ad6f0f7c6a0fa0f74.\r Table S2: Blast hit table. Primary and alternate assemblies were blasted against termini of the h-fibroin of Hesperophylax occidentalis to identify the h-fibroin in the respective assemblies.\r Figure S1: Genomescope Profile of kmers derived from HiFi reads (Enoicyla pusilla).\r Figure S2: Genomescope Profile of kmers derived from HiFi reads (Philocasca rivularis).\r Figure S3: Repeat landscape plot, where the x axis indicates divergence from consensus in Kimura distance, and the y axis indicates the percentage of the genome annotated as TE for each level of divergence. Ancient activity (greater divergence to consensus) appears on the left-hand side, while more recent activity is shown towards the right (greater similarity to consensus).\r Note S1: Schematic representation of the h-fibroin of Enoicyla pusilla.\r Note S2: Schematic representation of the h-fibroin of Philocasca rivularis.""" ; dct:issued "2026-04-20T12:53:00.854364"^^xsd:dateTime ; dct:modified "2026-04-21T10:05:53.870047"^^xsd:dateTime ; dct:title "Genomics of Caddisfly (Insecta: Trichoptera) Species Associated With Terrestrial Habitats" ; dcat:accessURL . a foaf:Organization ; foaf:name "SF - Terrestrial Zoology" . a schema1:PublicationEvent ; schema1:identifier "doi:10.1002/jez.b.23301" ; schema1:url "https://doi.org/10.1002/jez.b.23301" . a schema1:PublicationEvent ; schema1:identifier "doi:10.1007/978-1-0716-3838-5_16" ; schema1:url "https://doi.org/10.1007/978-1-0716-3838-5_16" . a schema1:PublicationEvent ; schema1:identifier "doi:10.1016/j.isci.2023.107253" ; schema1:url "https://doi.org/10.1016/j.isci.2023.107253" . a schema1:PublicationEvent ; schema1:identifier "doi:10.1016/j.tig.2025.01.004" ; schema1:url "https://doi.org/10.1016/j.tig.2025.01.004" . a schema1:PublicationEvent ; schema1:identifier "doi:10.1073/pnas.2221528120" ; schema1:url "https://doi.org/10.1073/pnas.2221528120" . a schema1:PublicationEvent ; schema1:identifier "doi:10.1093/g3journal/jkae093" ; schema1:url "https://doi.org/10.1093/g3journal/jkae093" . a schema1:PublicationEvent ; schema1:identifier "doi:10.1002/ece3.73327" ; schema1:url "https://doi.org/10.1002/ece3.73327" . [] a schema1:Taxon ; schema1:name "Philocasca rivularis" ; schema1:taxonRank "Species", "http://rs.gbif.org/vocabulary/gbif/rank/species" . [] a schema1:Taxon ; schema1:name "Lepidoptera" ; schema1:taxonRank "Order", "http://rs.gbif.org/vocabulary/gbif/rank/order" . [] a schema1:Taxon ; schema1:name "Trichoptera" ; schema1:taxonRank "Order", "http://rs.gbif.org/vocabulary/gbif/rank/order" . [] a schema1:Taxon ; schema1:name "Limnephilidae" ; schema1:taxonRank "Family", "http://rs.gbif.org/vocabulary/gbif/rank/family" . [] a schema1:Taxon ; schema1:name "Hyposmocoma kahamanoa" ; schema1:taxonRank "Species", "http://rs.gbif.org/vocabulary/gbif/rank/species" . [] a schema1:Taxon ; schema1:name "Elophila obliteralis" ; schema1:taxonRank "Species", "http://rs.gbif.org/vocabulary/gbif/rank/species" . [] a schema1:Taxon ; schema1:name "Enoicyla pusilla" ; schema1:taxonRank "Species", "http://rs.gbif.org/vocabulary/gbif/rank/species" .